By Mauro Bonazzi, Carlos Levy, Carlos Steel
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Extra resources for A Platonic Pythagoras. Platonism and Pythagoreanism in the Imperial Age
The m/z 171 diagnostic ion results from the collision-induced cleavage at the ureide bond of the AccQ·Tag adduct of each amino acid. 7. Since creating MRM-MS methods is costly and time consuming, the optimal cone voltage and collision energy parameters for each MRM transition were found only for a selected number of AccQ·Tag -amino acid adducts. , polar, nonpolar, acidic, basic, and neutral) were selected. The average values of cone voltages and collision energies observed for the evaluated amino acids were applied for the remaining targeted compounds through all the experiments.
LAA-21). 3. Amino acid stock solutions (1 mg/mL): Prepare 1 mg/mL solutions of individual L-amino acids in LC-MS grade water. 4. Derivatized amino acid solutions for infusions (10 μg/mL): Prepare 100 μg/mL solution of each L-amino acid by appropriate dilution of its corresponding stock solution. Derivatize each amino acid using the AccQ·Tag derivatization kit. Final 2 Combination of an AccQ·Tag-Ultra Performance Liquid… 17 amino acid concentration after derivatization is 10 μg/mL (see Note 4 and 5).
10. Stable-isotope-labeled reference compounds (internal standards): L-asparagine-15-N2; L-serine,2,3,3-d3; L-glutamine-2,3,3,4,4-d5; glycine-d5; D-L-alanine-2,3,3,3-d4; proline-2,5,5-d3; methioninemethyl-d3; tryptophan-2¢,4¢,5¢,6¢,7¢-d5(indole-d5); leucine-d10; valine-d8; L-histidine (ring 2-13C); L-glutamic acid-2,4,4-d3; ornithine-3,3,4,4,5,5-d6; lysine-3,3,4,4,5,5,6,6-d8; phenyl-d5alanine (Cambridge Isotope Laboratories, Andover, MA, USA and CDN isotopes, Pointe-Claire, Quebec, Canada). 11.
A Platonic Pythagoras. Platonism and Pythagoreanism in the Imperial Age by Mauro Bonazzi, Carlos Levy, Carlos Steel